Protein Engineering: Methods and Protocols

Protein Engineering: Methods and Protocols This volume details basic and advanced protocols for both stages of protein engineering: the library design phase and the identification of improved variants by screening and selection. Chapters focus on enzyme engineering using rational and semi-rational approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Protein Engineering: Methods and Protocols aims to aid scientists in the planning and performance of their experiments.The chapter 'Functional Analysis of Membrane Proteins Produced by Cell-Free Translation' is open access under a CC BY 4.0 license via link.springer. Autor: Uwe T. Bornscheuer, Matthias Hohne Wydawnictwo: Springer Nature EN Rok wydania: 2018 Okładka: miękka Liczba stron: 350 Wymiary: 25.4 x 17.8 x 1.5 cm Język: angielski ISBN: 9781493984633