Genetically Incorporated Non-Canonical Amino Acids: Methods and Protocols

Genetically Incorporated Non-Canonical Amino Acids: Methods and Protocols This detailed volume explores non-canonical amino acids (ncAAs) through their site-specific incorporation by genetic code expansion (GCE). The collection provides a broad resource of methods for implementing GCE in E. coli, mammalian cells, and animals, highlighting specific applications ranging from fluorescence labeling to photocontrol and the study of protein post-translational modification. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Genetically Incorporated Non-Canonical Amino Acids: Methods and Protocols serves as an ideal source of methodologies that can be adapted and extended, migrated to different model systems, and combined in new ways to help explore a wide range of biological questions and to augment industrial and pharmaceutical protein engineering. Wydawnictwo: Springer-Verlag New York Inc. Rok wydania: 2023 Okładka: miękka Liczba stron: 286 Wymiary: 25.4 x 17.8 cm Ilustracje: 1 Illustrations, black and white; XI, 286 p. 1 illus. Język: angielski ISBN: 9781071632536